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overview
Biology 571 is concerned with recent developments in the identification
and characterization of proteins using high-sensitivity, high-resolution
mass spectrometric (MS) techniques. Several new MS technologies,
including matrix-assisted laser desorption/ionization (MALDI),
electrospray ionization (ESI) and fast atom/ion bombardment (FAB)
are making the study of biomolecules routine. These technologies
enable the analysis of proteins, peptides, carbohydrates, oligonucleotides
and drug metabolites at the femtomole to picomole level with
a mass accuracy of + 0.001-0.01%. Working from the original literature
(and some work that has yet to be published), this course will
emphasize how, in what is now the post-genomic functional genomic
era, this technology has given rise to and sustains proteomics
in all its guises, from the study of single molecules to the
temporal and spatial definition of the total protein complement,
the 'proteome', of a cell.
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professors
teaching assistants
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course information
textbook
Introduction to Proteomics. Tools for the New Biology, Liebler,
D.C. (2001), Humana Press, Inc., Totowa, NJ, ISBN 0-89603-992-7.
grading and exams
Students will be assessed on the basis of short seminars that
they lead using material
from the original literature, participation in group discussions
and a short essay they
write highlighting, explaining and analyzing an original paper
from the field of
proteomics. For the essay, the students are asked to imagine
they have been invited to
write a short report, an "Opinion" or "Research
Update" article, for a high-profile journal
such as Nature or Science or a biotechnologically-biased journal
on an original paper
from the field of proteomics that was published in the last
12-18 months. Their
objectives will be to explain the principles underlying the
work, the general applicability
of the methods described and the conclusions drawn, what they
would like to do next if
they were working in the area concerned (perhaps they are already),
where there are
(or might be) interpretative ambiguities, and where the work
might take us in the years
to come. Students are advised to keep the length of the text
to 5 pages or less, single
space, Times 12 font (or equivalent) excluding figures (if
they choose to use them)
and/or bibliography (this is all the Editors-in-Chief will
allow!) (and students should not
think that because they have been offered 5 pages they must
use them all to do it well).
We are looking for a concise report that is not only instructive
and insightful but also fun
to read. The deadline for this report is Wednesday April 28,
4.00 pm.
lecture schedule
Please refer to the BIOL571 PDF
talk schedule for more information on this course.
academic integrity
All relevant University policies regarding Academic Integrity
must be followed. This includes no cheating, no plagiarism and
reporting any knowledge thereof. Please consult the Student
Handbook or the appropriate web-page: http://www.college.upenn.edu/responsibilities/integrity.html
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downloads
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course updates
01/13/2004
Other Information
Page was created today and is ready for updates.
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